合作類型：技術(shù) 行業(yè)類別: 化學(xué)與化學(xué)工程 過期時間：2018年03月08日     

一家斯洛文尼亞的研究機構(gòu)開發(fā)了一項有效的降解蛋白質(zhì)、蛋白質(zhì)聚合物并且使用熱穩(wěn)定的絲氨酸蛋白酶沉淀的技術(shù)。該方法可用于醫(yī)用手術(shù)設(shè)備的消毒、衣物清潔，并且在分子生物上，在各種溫度和酸堿度情況下都可以有效的洗滌。該研究機構(gòu)尋找合作伙伴進行許可證協(xié)議合作以及技術(shù)合作。

Summary:

A Slovenian research institute has developed an efficient method for degradation of proteins, proteins aggregates and deposits by using a thermally stable serine protease. The method is applicable for sterilization of surgical equipment in hospitals, cleaning of textiles, and in molecular biology protocols, and is efficient in a broad range of temperatures, pH values, and in presence of detergents. The researchers seek license or technical cooperation agreements.

Details:

Hospital equipment and surfaces contaminated with prions represent a constant health risk since prion proteins are notoriously resistant to high temperature and aggressive detergent treatments, making it difficult to sterilize such equipment using standard procedures. Moreover, there is no effective way to clean protein films in water pipes. There is need of a new, efficient, and environmentally friendly way of inactivation, elimination, and/or degradation of prion proteins. Currently used methods for prion degradation include application of extreme conditions.

The core of the invention solves these problems by introducing a simple and efficient protein degradation method using a thermally stable protease pernisine, obtained from the organism Aeropyrum pernix. The invention further includes a procedure in which pernisine is produced (expressed) in a common laboratory bacterium Escherichia coli (E. coli), and the protein gene sequence is specifically modified to allow for higher yields. The recombinant pernisine, purified from the lysed culture supernatant, is effective against soluble proteins as well as protein deposits.

Recombinant pernisines may be used (i) as replacement of proteinase K in molecular biology purification protocols (including kits); (ii) for sterilization of surgical equipment in hospitals; (iii) for cleaning of textiles (as component of washing powders and detergents); (iv) for removing allergenic peptides in food industry, or (v) for cleaning of solid surfaces with protein deposits (water pipes, bioreactor walls, etc.).

The researchers are among the leading scientists in their respective departments, and regularly publish in high-impact scientific journals. They are experts in the field of protein chemistry and biochemistry, extraction, purification, and isolation of proteins from thermophilic microorganisms, and protein stability studies.

Type and Role of Partners Sought:

- Type of partner sought: Industry, academy, research organisation,

- Specific area of activity of the partner: distribution of proteins, production of washing powders and cleaning products, sterilization of medical devices (surgical equipment)

- Task to be performed by the partner sought: development, production and commercialization of new products, sterilization of equipment, destroying protein deposits